Acetyl coenzyme A carboxylase system of Escherichia coli. Purification and properties of the biotin carboxylase, carboxyltransferase, and carboxyl carrier protein components.
نویسندگان
چکیده
The three protein components (biotin carboxylase, carboxyltransferase, and the biotin-containing carboxyl carrier protein of the acetyl coenzyme A carboxylase system have been resolved and purified extensively or to homogeneity from cell-free extracts of Escherichia co2i B. Carboxylation of acetyl-CoA to form malonyl-CoA requires the presence of all three components. Biotin carboxylase, which catalyzes the first half-reaction,
منابع مشابه
Biotin carboxyl carrier protein and carboxyltransferase subunits of the multi-subunit form of acetyl-CoA carboxylase from Brassica napus: cloning and analysis of expression during oilseed rape embryogenesis.
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Liver acetyl-CoA carboxylase, a biotin-enzyme which catalyzes the ATP-dependent carboxylation of acetyl-CoA (acceptor) to form malonyl-CoA (carboxylated acceptor), decarboxylates malonyl-CoA by a biotin-dependent, as well as a biotin-independent mechanism. Neither ADP, Pi, nor divalent metal ion are required for either of these abortive decarboxylations. The biotin-dependent reaction is blocked...
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ورودعنوان ژورنال:
- The Journal of biological chemistry
دوره 249 20 شماره
صفحات -
تاریخ انتشار 1974